Combination of rat tail collagen and platelet-derived growth factor-BB for culture of human umbilical vein endothelial cells

doi:10.3969/j.issn.2095-4344.2015.08.006

Abstract

Abstract:

BACKGROUND: Rat tail collagen type I can promote the increase of muscle fiber cells and migration and tube formation of endothelial cells, which is speculated to provide a more suitable internal environment for the growth of cells.

OBJECTIVE: To observe the effect and safety of platelet-derived growth factor-BB (PDGF-BB) with rat rail collagen against apoptosis of human umbilical vein endothelial cells in vitro.

METHODS: The passage 4 human umbilical vein endothelial cells were cultured in the medium of rat rail collagen and the reduction ratio in different time points was detected by Alamar Blue. The passage 4 human umbilical vein endothelial cells were divided into four groups and cultured in 24-well culture plates: control group, PDGF-BB group, H₂O₂ group, PDGF-BB+collagen group. H₂O₂ was used to induce cell apoptosis in all the groups. Western blot was used to detect the expression of PDGF-BB, apoptosis-related protein and anti-apoptosis protein after 72 hours. Meanwhile, TUNEL method was used to detect cell apoptotic rate.

RESULTS AND CONCLUSION: The tube formation in the human umbilical vein endothelial cells was more than that cultured in normal medium (P < 0.05). Cells cultured with rat tail collagen showed similar growth to normal control cells, indicating rat tail collagen had no cytotoxicity. The expressions of PDGF-BB, Bcl-2, and p-Akt in the PDGF-BB+collagen group were significantly higher than those in the other three groups (P < 0.05), but the expression of Bax was lower than that in the other three groups (P < 0.05). The apoptotic rate in the PDGF-BB+collagen group was lower than the PDGF-BB group and H2O2 group (P < 0.05). These findings indicate that rat tail collagen has no cytotoxicity to human umbilical vein endothelial cells, and rat tail collagen combined with PDGF-BB can predominantly enhance anti-apoptosis effects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Endothelial Cells, Collagen, Platelet-Derived Growth Factor

CLC Number:

R318

Cao Wen, Zhao Ai-chao, Chen Bang-dang, Liu Fen, Ma Yi-tong, Ma Xiang. Combination of rat tail collagen and platelet-derived growth factor-BB for culture of human umbilical vein endothelial cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(8): 1177-1183.

Figures/Tables 2

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